Diapause regulation in Rhagoletis pomonella and Rhagoletis cerasi
Diapause is a hormonally controlled seasonal response to environmental cues that results in developmental arrest, enabling arthropods to avoid unfavorable environmental conditions. Development and cell proliferation are halted during diapause until termination is triggered via environmental cues.
My current research focuses on understanding how diapause regulation differs in two populations of two species of Rhagoletis flies. Using a large scale comparative transcriptomic dataset through winter pupal diapause we are comparing gene expression changes between populations that terminate diapause earlier (apple hosts R. pomonella, low altitude R. cerasi) to populations that terminate diapause later (haw hosts R. pomonella, high altitude R. cerasi). Previous work (PI Greg Ragland) showed that by the end of winter gene expression differences are already pronounced between R. pomonella populations that infest apple and haw hosts (Meyers et al 2016). By examining gene expression differences through winter (from diapause initiation to near diapause termination) we will be able to build a clearer understanding of how diapause termination timing is controlled in early and late diapausing populations.
Meyers, PJ., Powell, THQ., Walden, KKO, Schieferecke, AJ., Feder, JL., Hahn, DA., Robertson HM., Berlocher, SH., Ragland, GJ. 2016. Divergence of the diapause transcriptome in apple maggot flies: winter regulation and post-winter transcriptional repression.